Highly Multiplexed Metabolic Engineering: A Shotgun Approach

A central goal of synthetic biology is to exert precise control over cellular behavior. In metabolic engineering, this is achieved through the coordination of multiple transcription units (TUs). The newly introduced shotgun genetic engineering (SGE) approach enables rapid discovery of complex metabolic pathways in mammalian cells, creating large-scale synthetic datasets. This method allows for the parallel screening of millions of pathway combinations, accelerating the pace of metabolic engineering.